pollen tube growth track and determination of compatibility in almond

نویسندگان

مهدی فلاح

دانشجوی سابق کارشناسی ارشد گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه شاهد، تهران، ایران یاور شرفی

استادیار گروه علوم باغبانی، دانشکدۀ کشاورزی، دانشگاه شاهد، تهران، ایران موسی رسولی

استادیار گروه مهندسی فضای سبز، دانشکدۀ کشاورزی، دانشگاه ملایر، همدان، ایران علی ایمانی

دانشیار، بخش باغبانی، مؤسسۀ تحقیقات اصلاح و تهیۀ نهال و بذر، کرج، ایران

چکیده

almond is one of the most important species of genus prunus. most almond cultivars and genotypes are self-incompatible and some of others are cross-incompatible. in this study compatibility and frequency pollen tube penetrate in the ovary were performed in three groups. the first group included the genotype a9.7 as female parent and tuono with genotypes a230, a10.11, a8.39 as male parents, the second group contained tuono as the female parents, the sahand cultivar with genotypes a230, a1.16 and a9.7 as male parents and third group included shokofeh cultivar as the female parent with genotypes sahand,tuono and a230, a1.16 as male parent were. 120 hours after controlled crosses, pistils fertilized with different pollens, isolated and the frequency of pollen tube growth was evaluated by florescence microscope in different parts of pistils. results showed that crosses ♂a9.7×♀ tuono (mean 3.33 pollen tube penetrates in the ovary) ♂a 8.39 ×♀ a9.7 (average 4.33, pollen tube penetrates in the ovary) and ♀a1.16 × ♀shokofeh (average of 4.33pollen tube penetrates in the ovary) highest number of pollen tubes penetrated to ovary and so they are very compatible for orchard establishment and crossing programs. with respect to compatibility and percentage of fruit set in crosses, to achieve optimum performance can of pollinizer a9.7, a1.16 and sahand in order to female parent tuono, a9.7 and shokofeh were used.

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